Inhibition of Transcription Induces Phosphorylation of YB-1 at Ser102 and Its Accumulation in the Nucleus
Résumé
The Y‐box binding protein 1 (YB‐1) is an RNA/DNA‐binding protein regulating gene
expression in the cytoplasm and the nucleus. Although mostly cytoplasmic, YB‐1 accumulates in
the nucleus under stress conditions. Its nuclear localization is associated with aggressiveness and
multidrug resistance of cancer cells, which makes the understanding of the regulatory mechanisms
of YB‐1 subcellular distribution essential. Here, we report that inhibition of RNA polymerase II
(RNAPII) activity results in the nuclear accumulation of YB‐1 accompanied by its phosphorylation
at Ser102. The inhibition of kinase activity reduces YB‐1 phosphorylation and its accumulation in
the nucleus. The presence of RNA in the nucleus is shown to be required for the nuclear retention
of YB‐1. Thus, the subcellular localization of YB‐1 depends on its post‐translational modifications
(PTMs) and intracellular RNA distribution.
| Origine | Publication financée par une institution |
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