Incorporation of 2,3-Diaminopropionic Acid into Linear Cationic Amphipathic Peptides Produces pH-Sensitive Vectors - Université d'Évry Access content directly
Journal Articles ChemBioChem Year : 2010

Incorporation of 2,3-Diaminopropionic Acid into Linear Cationic Amphipathic Peptides Produces pH-Sensitive Vectors

Yun Lan
  • Function : Author
Vincenzo Abbate
  • Function : Author
Xiaole Kong
  • Function : Author
Kelly Sullivan
  • Function : Author
Christian Leborgne
Robert Hider
  • Function : Author
Alex Drake
  • Function : Author
Sukhvinder Bansal
  • Function : Author
Antoine Kichler
a James Mason
  • Function : Author
Bérangère Lan
  • Function : Author
A. James Mason
  • Function : Author

Abstract

Nonviral vectors that harness the change in pH in endosomes, are increasingly being used to deliver cargoes, including nucleic acids, into mammalian cells. Here we present evidence that the pK(a) of the beta-NH(2) in 2,3-diaminopropionic acid (Dap) is sufficiently lowered, when Dap is incorporated into peptides, that its protonation state is sensitive to the pH changes that occur during endosomal acidification. The lowered pK(a) of around 6.3 is stabilized by the increased electron-withdrawing effect of the peptide bonds, by intermolecular hydrogen bonding and from contributions arising from the peptide conformation. These include mixed polar/apolar environments, Coulombic interactions and intermolecular hydrogen bonding. Changes in the charged state are therefore expected between pH 5 and 7, and large-scale conformational changes are observed in Dap-rich peptides, in contrast to analogues containing lysine or ornithine, when the pH is altered through this range. These physical properties confer a robust gene-delivery capability on designed cationic amphipathic peptides that incorporate Dap.

Dates and versions

hal-02179234 , version 1 (10-07-2019)

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Yun Lan, Vincenzo Abbate, Berangere Bertin, Louic Vermeer, Xiaole Kong, et al.. Incorporation of 2,3-Diaminopropionic Acid into Linear Cationic Amphipathic Peptides Produces pH-Sensitive Vectors. ChemBioChem, 2010, 11 (9), pp.1266-1272. ⟨10.1002/cbic.201000073⟩. ⟨hal-02179234⟩
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